mheiser98031 mheiser98031

26-01-2024
Biology

contestada

I want to load my agarose gel with my PCR products, which are 50 µL reactions.
I am wondering how much of those reaction I can load into one well if I use a standard agarose mini gel (10 well comb, ~8 mm thickness, 1.2%).
Should I try loading the entire 50 µL into one well or should I split it into two?
Thanks in advance
Phil

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